Development of Eco-friendly Iron Nanoparticles Biosensors Synthesized from Guiera senegalensis Stem-bark Extract for Biomolecule Detection

Authors

  • Mela Yoro Department of Chemical Sciences, Faculty of Science, Federal University of Kashere, P.M.B 0182, Gombe, Nigeria Author
  • Japhet Joshua Department of Science Laboratory Technology, Federal Polytechnique, Kaltungo, Gombe, Nigeria Author
  • Gamache Y. Markus Department of Science Laboratory Technology, Federal Polytechnique, Kaltungo, Gombe, Nigeria Author
  • Samuel Johnson Department of Science Laboratory Technology, Federal Polytechnique, Kaltungo, Gombe, Nigeria Author
  • Sufyan C. Salihu Department of Science Laboratory Technology, Federal Polytechnique, Kaltungo, Gombe, Nigeria Author

Keywords:

Nanoparticles, Biosensors, Guiera Senegalensis, Stem Extract, Biomolecules

Abstract

The development of eco-friendly nanomaterials for biomedical applications has gained significant attention in recent years. In this study, iron (Fe) monometallic nanoparticles were synthesized using Guiera senegalensis stem extract as a natural reducing and stabilizing agent. The green synthesis approach provides a sustainable and non-toxic alternative to conventional chemical methods, while also enhancing nanoparticle stability and biocompatibility. The structural and morphological features of the biosynthesized Fe nanoparticles were characterized using standard analytical techniques. These nanoparticles were further employed in the fabrication of a biosensor for the detection of biomolecules, with glucose serving as a model analyte. The Fe-based biosensor demonstrated high sensitivity, good selectivity, and a rapid response time toward glucose detection, highlighting its potential for biomedical diagnostics and environmental monitoring. This work underscores the promise of G. senegalensis-mediated Fe nanoparticles as a cost-effective and sustainable platform for next-generation biosensing applications.

References

Bachheti RK, Fikadu A, Bachheti A, Husen A. Biogenic Fabrication of Nanomaterials from Flower-based Chemical Compounds, Characterization and their various Applications: A review. Saudi J Biol Sci. 2020; 27(10):2551–2562.

Christian P., Von der Kammer F., Baalousha M., Hofmann T. Nanoparticles: Structure, properties, preparation and behaviour in environmental media. Ecotoxicology. 2008;17:326–343. doi: 10.1007/s10646-008-0213-1. [DOI] [PubMed] [Google Scholar]

Gui M., Smuleac V., Ormsbee L.E., Sedlak D.L., Bhattacharyya D. Iron oxide nanoparticle synthesis in aqueous and membrane systems for oxidative degradation of trichloroethylene from water. J. Nanopart. Res. 2012;14:1–16. doi: 10.1007/s11051-012-0861-1. [DOI] [PMC free article] [PubMed] [Google Scholar

Huq MA. Green synthesis of silver nanoparticles using Pseudoduganella eburnean MAHUQ-39 and their Antimicrobial Mechanisms Investigation against Drug-Resistant Human Pathogens. Int J Mol Sci. 2020; 21(4):1510

Sreeja V., Jayaprabha K.N., Joy P.A. Water-dispersible ascorbic-acid-coated magnetite nanoparticles for contrast enhancement in mri. Appl. Nanosci. 2014;5:435–441. doi: 10.1007/s13204-014-0335-0. [DOI] [Google Scholar]

Sun X., Zheng C., Zhang F., Yang Y., Wu G., Yu A., Guan N. Size-controlled synthesis of magnetite (Fe3O4) nanoparticles coated with glucose and gluconic acid from a single Fe(III) precursor by a sucrose bifunctional hydrothermal method. J. Phys. Chem. C. 2009;113:16002–16008. doi: 10.1021/jp9038682. [DOI] [Google Scholar]

Mela, Y., Japhet, J., Isiyaku, A. (2022). Green Synthesis, Characterization and Antimicrobial Potency of Ag–Fe Bimetallic Nanoparticles from Papaya Leaf Extract. International Journal of Scientific and Research Publications, 12(2):563-568

Anuradha Ch.S, Susheela Bai G., Kishore Babu B., Phyto Fabricated Green Synthesis of Novel Ag-Fe Bimetallic Nanoparticles : Characterization and Photocatalytic Activity on Malachite Green Dye, International Journal of Engineering Development and Research, (2020), 8(2): 2321-9939

A. L. Padilla‑Cruz1, J. A. Garza‑Cervantes, X. G. Vasto‑Anzaldo, Gerardo García‑Rivas, A. León‑Buitimea, & J. R. Morones‑Ramírez, Synthesis and design of Ag Fe bimetallic nanoparticles as antimicrobial synergistic combination therapies against clinically relevant pathogens, Science Report, (2021), 11:5351

Shailesh C Kotval, Kokila A Parmar, Tessy John, Green Synthesis of Silver Nanoparticles using Elytraria acaulis Plant Root Extract and its Antimicrobial activity, International Journal of Green and Herbal Chemistry, (2018), 7(3): 598-609.

A. Ruíz-Baltazar, R. Esparza, R. Pérez and G.Rosas, Structural Characterization of Fe-Ag Bimetallic Nanoparticles Synthesized by Chemical Reduction, International Research Journal of Pure & Applied Chemistry, (2014), 4(3): 263-269,

N Hikmah, N F Idrus, J Jai and A Hadi, Synthesis and characterization of silver-copper core-shell nanoparticles using polyol method for antimicrobial agent, International Conference on Chemical Engineering and Bioprocess Engineering, (2016), 36: 12-50

Anuradha Ch.S, Susheela Bai G., Kishore Babu B., Phyto Fabricated Green Synthesis of Novel Ag-Fe Bimetallic Nanoparticles : Characterization and Photocatalytic Activity on Malachite Green Dye, International Journal of Engineering Development and Research, (2020), 8(2): 2321-9939

A. L. Padilla‑Cruz1, J. A. Garza‑Cervantes, X. G. Vasto‑Anzaldo, Gerardo García‑Rivas, A. León‑Buitimea, & J. R. Morones‑Ramírez, Synthesis and design of Ag Fe bimetallic nanoparticles as antimicrobial synergistic combination therapies against clinically relevant pathogens, Science Report, (2021), 11:5351

Shailesh C Kotval, Kokila A Parmar, Tessy John, Green Synthesis of Silver Nanoparticles using Elytraria acaulis Plant Root Extract and its Antimicrobial activity, International Journal of Green and Herbal Chemistry, (2018), 7(3): 598-609.

Mela, Y., Rebeccah, B., Mary, A.M., Robinson, A.F(2024)."Eco-friendly Synthesis, Characterization, and Antimicrobial Study of Mono Metallic Silver Nanoparticles from Khaya senegalensis Aqueous Stem Bark Extract". International Journal of Scientific Research in Chemistry (IJSRCH), ISSN: 2456-8457, Volume 9, Issue 2, pp.01-09, (2024)

Manasreh, O. (2011). Introduction to Nanomaterials and Devices.

J, C. T., Shikha, S., & Ramesh, R. (2013). Nanotechnology: Interdisciplinary science of applications. African Journal of Biotechnology, 12(3), 219–226.

Sánchez-López, E. et al. Metal-based nanoparticles as antimicrobial agents: an overview. Nanomaterials 10, 292 (2020).

Arora, N., Thangavelu, K. & Karanikolos, G. N. Bimetallic nanoparticles for antimicrobial applications. Front. Chem. 8, 412 (2020).

Belenov, S. V. et al. Phase behavior of Pt–Cu nanoparticles with different architecture upon their thermal treatment. Nanotechnologies Russ. 12, 147–155 (2017).

Behera, A., Mittu, B., Padhi, S., Patra, N. & Singh, J. Bimetallic nanoparticles: Green synthesis, applications, and future perspectives. In Multifunctional Hybrid Nanomaterials for Sustainable Agri-Food and Ecosystems 639–682 (Elsevier, 2020). doi:https: //doi.org/10.1016/B978-0-12-82135 4-4.00025 -X

Sharma, G. et al. Novel development of nanoparticles to bimetallic nanoparticles and their composites: a review. J. King Saud Univ. - Sci. https ://doi.org/10.1016/J.JKSUS .2017.06.012 (2017).

Mela, Y., Amos, G., Japhet, J., & Ayuba, I. (2022). Green Synthesis, Characterization and Antimicrobial Potency of Silver Nanoparticles from Psidium guajava Leaf Extract. Online Journal of Chemistry, 2022(2): 14-22

Botha, T. L. et al. Cytotoxicity of Ag, Au and Ag–Au bimetallic nanoparticles prepared using golden rod (Solidago canadensis) plant extract. Sci. Rep. 9, 4169 (2019).

Sharma, V. K., Siskova, K. M. & Zboril, R. Magnetic Bimetallic Fe/Ag Nanoparticles: Decontamination and Antimicrobial Agents. In: ACS Symposium Series 1150, 193–209 (American Chemical Society, 2013).

Gallo, A., Bianco, C., Tosco, T., Tiraferri, A. & Sethi, R. Synthesis of eco-compatible bimetallic silver/iron nanoparticles for water remediation and reactivity assessment on bromophenol blue. J. Clean. Prod. 211, 1367–1374 (2019).

Akter S, Huq MA. Biologically Rapid Synthesis of Silver Nanoparticles by Sphingobium sp. MAH-11 T and their Antibacterial Activity and Mechanisms investigation against Drug-resistant Pathogenic Microbes. Artif Cells Nanomed Biotechnol. 2020; 48 (1):672–682

Downloads

Published

19-11-2025

Issue

Section

Research Articles

How to Cite

[1]
Mela Yoro, Japhet Joshua, Gamache Y. Markus, Samuel Johnson, and Sufyan C. Salihu, “Development of Eco-friendly Iron Nanoparticles Biosensors Synthesized from Guiera senegalensis Stem-bark Extract for Biomolecule Detection”, Int J Sci Res Chemi, vol. 10, no. 6, pp. 01–07, Nov. 2025, Accessed: Dec. 02, 2025. [Online]. Available: https://mail.ijsrch.com/index.php/home/article/view/IJSRCH251061